Frequency and molecular characterization of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in neonatal sepsis, Peru

Abstract

Background: Acinetobacter (A.) baumannii is a gram negative on-fermentative occobacilli with increasing relevance in a varity of hospital-acquired infections. The bacterium is a nosocomial pportunistic pathogen that are resistant to the most of antibitics and causes various types of infections such as bacterimia, neumonia, meningitis and urinary tract infections. The aim of his study was to determine the frequency of bla-OXA-2 and blaXA-10 resistance genes and class I Integron among Acinetobacter aumannii strains isolated from patients referred to Imam Reza ospital of Tabriz city. Methods: A total of 100 clinical isolates of A. baumannii were ollected from patients who referred to Imam Reza Hospital of abriz. The isolateswere identified using standard Biochemical and icobiological tests. ESBLproductionwasdetermined in isolatesby ouble disk synergy test. The presence of bla-OXA-10, bla-OXA-2 nd INT1 genes in clinical isolates were investigated by PCR techique. Results: The results of antimicrobial sensitivity tests revealed hat the highest resistance was against cefiexim (100%), ceftioxim(100%) and ticaracilin (100%), whereas the highest suscepibility was observed for polymixin B (84%), colistin (77%) and efampin (73%). Double Disk Synergy Test showed that 60% of isoatedwereESBLproduceramong them7cases (11.6%)werepositive or bla-OXA-2 and 5 cases (8.3%) for bla-OXA-10 genes. Screening or Int-1 genes demonstrated that 75.2% of isolates were positive or Int-1 insertion sequence. Conclusion: The results of the present study indicated that XA-2andOXA-10 typeESBLshavenoticeableprevalence inAcineobacter baumannii in the study region and it is necessary to onsider appropriate preventive measures to avoid further spread f such resistant strains.