Frequencies of genotypes and alleles of rs41256848 in the LHCGR gene in the population of black-and-white holsteinized cattle

Abstract

Embryo transfer technique is one of the key in accelerated reproduction of cattle. One of the most important stages is the selection of donor cows that are most sensitive to the procedure of hormonal stimulation of ovulation. One of the promising genetic markers of the reproductive status of cattle is currently the gene encoding the luteinizing hormone / choriogonadotropin receptor (LHCGR). One of the SNP in the LHCGR gene of cattle has already been described in the literature as associated with the number of oocytes and the quality of embryos - rs41256848 (c.1401G> T, p.Trp467Cys). The purpose of this work was to estimate the frequencies of genotypes and alleles of this substitution in the population of Black-and-White holsteinized cattle (190 cows). Genotyping was performed by PCR-RFLP method. In the studied population of cattle, the frequency of the G allele associated with higher rates in the total number of oocytes and the number of embryos survived after transplantation, as well as with the least number of unfertilized oocytes, is 63.2%.