Freezing of washed Angora goat semen with extenders added bull or ram seminal plasma.

Abstract

Summary The aim of this study was to investigate necessity of separation of Angora goat seminal plasma and beneficial of supplementation of bovine or ovine seminal plasma (SP) to extender prior to semen cryopreservation. Ejaculates from 4 Angora Goats were used in this study. Four experimental groups were designed: T (Angora Goat Semen (AGS) washed with Tyrod’s solution with hepes (TALP), extended with TRIS containing egg yolk (TEG); C (Control, AGS not washed, only extended with TEG); TO (AGS washed with TALP, extended with TEG containing ovine seminal plasma (SP); TB (AGS washed with TALP, extended with TEG containing bovine SP). When post-thaw AGS parameters of four groups were analyzed, it was attained that supplementation of bovine or ovine seminal plasma to extender improved post-thaw AGS parameters compared with C and T groups (P<0.05). In conclusion, the results of the current study suggest that AGS should be washed. Moreover, the results of this study point out that supplementation of bovine or ovine SP instead of Angora goat SP separated from semen can be used prior to freezing.