Abstract
We aimed to determine the optimal cryopreservation method for eradication of the greater wax moth (Galleria mellonella) from honey bee comb. To pursue this goal, mortality rates of G. mellonella eggs, larvae, pupae and adults were studied after treatment at −15 or −20 °C for varying durations. The results showed that larvae, pupae and adults all died after they were treated for one hour at either −15 or −20 °C. Moth eggs, on the other hand, had strong frost resistance. It was only when the treatment time increased to 10 h that all G. mellonella eggs were killed. Freeze tolerance of 7-day-old eggs was higher than that of 1, 3 and 5 day-old eggs. We propose two protocols for cryopreservation of comb to kill all stages of the greater wax moth.
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