Abstract

Two freezer-independent methods for the preservation of soluble carbohydrates in macrophyte tissues were tested against fresh as well as frozen controls, the latter jointly analysed after storage for up to 4 weeks. Eight soluble monosaccharides and oligosaccharides were covered, including sucrose, fructose and glucose, in photosynthetic tissues of six species of aquatic macrophytes. Fixation in 5% formalin did not prove satisfactory, while sterilisation at 110°C after extraction did. Extraction and sterilisation can both be done by relatively simple means. When reliable availability of electric power cannot be taken for granted, e.g. in remote areas or during long-distance transport, such methodology will prove its usefulness.

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