Abstract
We have demonstrated that the “rippled” appearance which is seen occasionally in lamellar body (LB) membranes is a temperature-dependent phenomenon and probably represents crystallization of the phospholipids. The “rippled” pattern is a consistent finding when lung has been cooled to 4°C before quenching into Freon. It is rarely observed in lamellar bodies quenched from room temperature. The ripples have been seen in a preparation of isolated lamellar bodies. The presence of glycerol as a cryoprotectant has no effect on this phenomenon. Fixation at low temperature does not preserve the “ripple” pattern when specimens are raised to 22°C prior to quenching. Comparison with freeze-fracture pictures of synthetic phospholipid membranes in the literature suggests that the “rippled” lamellar body membranes are in a Pαβ state.
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