Abstract
The concave and convex fracture faces of the inner and outer membranes of rat liver mitochondria have been studied. The patchwork pattern previously described from preparations of whole mitochondria was concluded to result from cleavage through sections of the outer membrane. The observed particle densities of concave and convex fracture faces of the inner and outer mitochondrial membranes differ and this difference may have functional significance. Thus, further studies of mitochondrial membrane surfaces using the technique of freeze-fracture will necessitate a prior separation of the inner membrane from the outer membrane.
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