Freeze-fracture characteristics of insect gustatory and olfactory sensilla. I. A comparison with vertebrate olfactory receptor cells with special reference to ciliary components.

Abstract

Freeze-fracture data on antennal olfactory and labellar gustatory sensilla of the blowfly Calliphora vicina were compared with those of vertebrate olfactory organs. Insect antennal and vertebrate olfactory axons have similar diameters and show vesicular expansions; insect labellar axons are on average twice as thick and show no vesicular expansions. Vertebrate olfactory and insect labellar and antennal axons display similar intramembranous particle densities. Antennal axons show particle arrangements, resembling tight-junctions. The few extremely thick axons found in labella and antennae show particle arrangements resembling gap-junctions. In regions, proximal to the pores in the insect sensillar hairs, P-faces of olfactory and gustatory cilia show about 200 particles/microns2. The most proximal and distal portions of the sensory cilia, necklaces and regions in the vicinity of the hair pores respectively, were only encountered in antennal sensilla. P-faces of the ciliary membranes underneath these pores display 1,000-1,200 particles/microns2 in unbranched and branched cilia. These values agree with values found in vertebrate olfactory cilia. It is suggested that these high particle densities are related to entities involved in chemoreceptive activities. Accessory cell micropliae have P-face densities of 2,000-3,000 particles/microns2, values similar to those found in vertebrate supportive cell microvilli. The membranes of the accessory cells display septate-junctions in areas where these cells overlap themselves, each other and in places where they adhere to the exoskeleton or the basement membrane.