Abstract
Yoshikawa H. & Hayakawa A. 1996. Freeze-fracture cytochemistry of membrane cholesterol in Blastocystis hominis. International Journal for Parasitology 26: 1111–1114. Membrane cholesterol in Blastocystis hominis was detected by freeze-fracture methods using a polyene antibiotic, filipin. Since the intramembrane particles (IMP) were distributed heterogeneously on both plasma and vacuole membranes, many IMF-free areas were observed. Even in filipin-treated cells, filipin-cholesterol complexes were not detected in IMF-free areas on the plasma membrane, whereas on the central vacuole membrane the complexes were mainly observed in IMF-free regions. These results indicate the different organization of the membrane cholesterol between the plasma membrane and central vacuole membrane. Most of the granules in the central vacuole were densely labeled with filipin, indicating the accumulation of cholesterol in the vacuole.
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