Abstract
Stumpf and Roth (1967) designed an apparatus for tissue freeze drying by cryosorption pumping. Under the conditions they used, the capacity was virtually limited to tissue samples of 10 mg or less. Freeze drying with a new molecular sieve (Linde 13 × 1/16; inch) and a tissue temperature of -50 C however dries tissue samples weighing up to 500 mg in 3 days. The all-glass freeze drying unit (figure 1) is slightly modified for vapour fixation and embedcling at 60-90 C compared with that of Stumpf and Roth.
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