Abstract
A freeze-drying and shadowing procedure for two-dimensional periodic biological structures that allows the subsequent application of image processing techniques is reported. Conditions for shadow-casting (shadowing geometry, deposit thickness) have been adjusted such that optically filtered images from shadowed specimens can be related to those from negatively stained preparations of the same structure. Our test specimen did not show systematic deformations of the fine structure after freeze-drying when compared to its stain exclusion pattern. Under optimal conditions the resolution was 25–30 A as judged from the highest diffraction orders which still carried recognizable structural information in the filtered image. Applications of this freeze-drying and shadowing procedure combined with image processing are discussed within a larger scope.
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