Abstract
The occurrence of free and glycosidic vitamin D 3 and its metabolises in Cestrum diurnum leaves was investigated. The chloroform extract of the leaves (containing free vitamin D 3 and its metabolites) and the chloroform-methanol (1:2) extract (containing glycosidic vitamin D 3 and its metabolites) of the residue after glycosidase treatment were partially purified by column chromatography. Fractions corresponding to authentic vitamin D 3, 25-hydroxy vitamin D 3 (25-OH-D 3) and 1,25-dihydroxy vitamin D 3 (1,25-(OH) 2D 3) were found to be biologically active. These fractions were also analysed and quantified by HPLC. This is the first demonstration of the presence of free vitamin D 3, 25-OH-D 3 and 1,25-(OH) 2D 3 in a carcinogenic plant. Apart from the free forms, the corresponding glycosidic metabolites were also found. The concentration of free metabolises was much higher than that of the glycosidic forms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
