Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice

Raju Asirvatam,Ajm Christina

doi:10.14499/indonesianjpharm29iss3pp136

Abstract

Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper ( Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper ( Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K (-) (Na-CMC 0.5%), K (+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D (1) (3.33mg/kg BW), D (2) (6.66mg/kg BW) and D (3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in male rat.

Highlights

Oxidative stress is characterized by an increased level of reactive oxygen species (ROS) that disrupts the intracellular reductionoxidation balance and has been implicated in various diseases including cancer
After treatment with the doses of 250, 500mg/kg of ethanol extract of D.indica (EEDI) and aqueous extract of D.indica (AEDI) was significantly increased the free radical defense enzyme level more or less like normal groups when compared with Dalton Ascites lymphoma (DAL) control mice
The protein carbonyl content (PCC) content in hemolysate was increased in DAL control mice

Summary

Introduction

Oxidative stress is characterized by an increased level of reactive oxygen species (ROS) that disrupts the intracellular reductionoxidation (redox) balance and has been implicated in various diseases including cancer. Antioxidants can inhibit or delay the oxidation of oxidizable substrates and this appears to be very important in the prevention of oxidative stress which is suggested as the leading cause of many oxidation related diseases (Sara et al, 2012). Phytochemicals such as phenolics, carotenoids and flavonoids, have been shown to possess functional properties such as antimicrobial, antimutagenic, and free radical scavenging activity. These phytoconstituents acts as a natural antioxidant and prevent the free radical formation (Anil et al, 2013). Under the natural category one of the flavonoid rich plant is Drosera

Objectives

Methods

Results

Conclusion