Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (Prunus domestica L.) in both fresh and dried samples.

Abstract

Consumption of fruits, such as plums and prunes, is useful in treating blood circulation disorder, measles, digestive disorder, and prevention of cancer, diabetes, and obesity. The paper presents a description of antioxidant and antiradical capacity of plum (Prunus domestica L.) in both fresh and dried samples. Samples were mixed with methanol and ethanol (as solvents) and were extracted on magnetic shaker, separately. The experiments were carried out to measure the Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC), Reducing Power Assay (RPA), Chain Breaking Activity (CBA), and quantity of Malondialdehyde (MDA), 2,2-Diphenyl-1-Picrylhydrazyl (DPPH),Nitric Oxide (NO),Hydrogen peroxide (H2O2) and superoxide(O2 (-)) radicals inhibition. The results showed that the highest values for the TPC, TFC,TAC, RPA, CBA, DPPH, and NO were related to ethanolic extractsof dried sample which showed statistically significant differences (p<0.01 and p<0.0001), while the maximum values for the H2O2 and O2 (-)were related to ethanolic extracts of fresh sample. The correlations data were analyzed among all parameters and the TPC and TFC had a significant correlation (r(2)=0.977). Moreover, it was found that methanol was more successful in extraction procedure than ethanol (p<0.01). Findings suggest that the fresh samples are more successful in collecting oxygen free radicals such as superoxide (O2 (-)) and peroxy radicals (ROO(.)) than dried.