Free radical scavenging and radioprotective effects of carnosine and anserine

Abstract

Two histidine-containing natural dipeptides, carnosine and anserine (β-alanyl-1-methyl- l-histidine), have been examined for their antioxidant and radioprotective abilities. Pulse radiolysis studies indicated the antioxidative properties of carnosine and anserine aqueous solutions at different pH. The rate constants for the reaction OH radical with carnosine at neutral pH were determined to be 5.3×10 9 M −1 s −1 at 300 nm, and 4.1×10 9 M −1 s −1 at 400 nm, respectively. Carnosine and anserine also protected plasmid pUC18 DNA from X-ray radiation-induced strand breaks as evidenced from the studies by agarose gel electrophoresis. Carnosine showed higher protective efficiency under the experimental conditions. Our data demonstrated that carnosine and anserine may play an important role in the maintenance of the antioxidant system.