Abstract
Oxidative stress is associated with the pathogenesis of diabetes and obesity. The key enzymes involved in the carbohydrate and lipid metabolisms are a-amylase and pancreatic lipase respectively. The phytochemicals present in medicinal plants are known to be potent free radical scavenging agents and great inhibitors of metabolizing enzymes. Phyllanthus amarus leaves have antioxidant, anti-diabetic, and anti-obesity properties. This research is aimed at elucidating the carbohydrate and lipid metabolism inhibitory activities of P. amarus leaves through in-vitro screening. The phytochemical screening revealed that P.amarus is having high phenolic, flavonoid, tannin, and saponin content. The total protein content of P.amarus showed 2.720±0.001 mg BSAE/g. Diphenyl-2-picrylhydrazyl (DPPH) assay showed 0.0001mg/mL, Nitric oxide assay revealed 0.452 mg/mL, Ferric Reducing Antioxidant Power (FRAP) assay showed a value of 0.132±0.010 mg RE/g, Total antioxidant capacity (TAC) indicated a value of 2.217±0.002 mg GAE/g and 2,2-azino-bis(3-ethylbenzotiazoline)-6-sulfonic acid (ABTS) inhibitory activity at the peak concentration (1.2 mg/mL) was 40.29%. The enzyme inhibitory assessment showed a decrease in a-amylase activity as the concentration increased while there was a slight decrease in a-lipase activity but a slight increase at 18.75 mg/mL.
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