Abstract
Antioxidant activity of the extract from the barks of Ilex rotunda was investigated using electron spin resonance (ESR) spectroscopy on 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, hydroxyl, and superoxide radicals. The extract exhibited strong free radical scavenging activity with scavenging percentages of DPPH radical (82.2 ± 1.8%), alkyl radical (82.9 ± 1.7%), hydroxyl radical (89.9 ± 2.1%), and superoxide radical (92.5 ± 2.6%) at concentration of 50 μg/ml, respectively. Activity increased with increasing extract concentrations. Therefore, these results presented the extract from the barks of I. rotunda with a potent antioxidant activity that could be useful in food, pharmaceutical, and cosmetic industries. Key words: Ilex rotunda, antioxidant, free radical scavenging activity, electron spin resonance.
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