Abstract
The antioxidative activity of extracts from Juniperus rigida Sieb. Fruit (JRSF) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The free radical scavenging activity of the extract was dose dependant manner, and the IC50 value on DPPH, hydroxyl and alkyl radical at 16.84, 82.35 and 52.27 μg/mL respectively. In addition, evaluated the protection effect on H2O2-induced oxidative damage in PC-12 cells via propidium iodide (PI) staining using a flow cytometer. JRSF extract decreased cell death in PC-12 cells due to H2O2-induced oxidative damage in a dose-dependent manner. These results suggest that JRSF extract exhibit antioxidative activity against oxidative stress on PC-12 cells.
Highlights
Reactive oxygen-mediated modification of DNA, proteins, lipids and small cellular molecules is associated with a number of pathological processes, including atherosclerosis, arthritis, diabetes, cataractogenesis, muscular dystrophy, pulmonary dysfunction, inflammatory disorders, ischemia-reperfusion tissue damage and neurological disorders such as Alzheimer’s disease [1]
Hydroxyl radicals generated in the Fe2+/H2O2 system were trapped by Dimethyl-1-pyrroline N-oxide (DMPO), forming a spin adduct detected by an electron spin resonance (ESR) spectrometer
The alkyl radical spin adduct of 4-POBN/free radicals was generated from azobis (2-amidinopropane) hydrochloride (AAPH) at 37°C for 30 min, and the decrease of ESR signals was observed with the dose increment of the extract from Juniperus rigida Sieb. Fruit (JRSF). alkyl radical scavenging activities of the extract from JRSF were 36.50, 56.11 and 88.66% at 31.25, 62.5 and 125 μg/mL, respectively, and the IC50 value was 52.27 μg/mL (Figure 1C)
Summary
Reactive oxygen-mediated modification of DNA, proteins, lipids and small cellular molecules is associated with a number of pathological processes, including atherosclerosis, arthritis, diabetes, cataractogenesis, muscular dystrophy, pulmonary dysfunction, inflammatory disorders, ischemia-reperfusion tissue damage and neurological disorders such as Alzheimer’s disease [1]. Antioxidants can act at different levels in an oxidative sequence This may be illustrated by considering one of the many mechanisms by which oxidative stress can cause damage by stimulating the free radical chain reaction of lipid peroxidation [3]. Free radical chain reactions within a material could be inhibited by adding chemicals that retard the formation of free radicals, by introducing substances that retard the formation of free radicals or by introducing substances that compete for the existing radicals and remove them from the reaction medium [3] Many synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tertiary-butylhydroquinone (t-BHQ) and propyl gallate (PG) may be used to retard lipid peroxidation in a lot of fields [3,4]. The present study aimed to investigate the free radical scavenging activities of extracts from JRSF by ESR spectroscopy and their possible protective effects on PC-12 cells against oxidative stress
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
