Abstract
(Sorensen buffer, pH7.4, 37°C) with (a) cocaine, morphine and methadone, (b) with the oxidative enzymes, and (c) with cocaine, morphine and methadone in the presence of oxidative enzymes. The oxidative enzymes were PHS purified from ram seminal vesic1es (20 U/mg), and SIO Mix liver microsomes from phenobarbital treated rats (4 mg/mi protein). The reaction was stopped by chilling the tubes at 0-4 oe. After centrifugation, histamine and lactate dehydrogenase (LDH) were analysed in the supernatants and in the pellets (fluorimetric and spectrophotometric assays, respectively) and expressed as percent of the total histamine content. MDA was measured by a spectrophotometric assay [4].
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
