Abstract
Free radicals in thermally treated chloramphenicol were examined by electron paramagnetic resonance (EPR) spectroscopy. The parameters and shape of EPR spectra were analysed and free radical concentrations were obtained in the tested drug samples. Chloramphenicol was thermally sterilized at pharmacopeia conditions: 100 °C (120 min). Sterilization was also carried out at different conditions, 110 °C (60 min) and 120 °C (30 min), for comparison with pharmacopeia settings. Microbiological analysis was performed on the samples to confirm sterility. The aim of this work was to determine the concentration of free radicals in chloramphenicol following thermal sterilization at pharmacopeia conditions and compare this with other sets of conditions [110 °C (60 min) and 120 °C (30 min)]. The best conditions of thermal sterilization are determined as those that kill microorganisms and produce the lowest amounts of free radicals in this drug. It was concluded that the optimal temperatures and times for the thermal sterilization of chloramphenicol are 100 °C and 120 min and 110 °C and 60 min. A temperature of 120 °C coupled with a heating time of 30 min was rejected for thermal sterilization because of the high amount of free radicals produced by the drug samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
