Abstract
Microfluidic free flow electrophoresis (FFE) enables several advantages directly related to size reduction, however at the cost of difficult fabrication strategies that compromise either the device's efficiency or shelf-time. We circumvent these limitations by integrating polycarbonate sheets as ionic permeable membranes to separate electrolyte and separation chamber compartments. The membrane effectively blocks hydrodynamic flow while no additional electrical resistance between fluidic compartments could be measured. We performed isoelectric focusing (IEF) experiments to separate a series of proteins with different pI values in the 3.5-9.3 range. We also demonstrate the separation of DNA according to its size in zone electrophoresis (ZE) mode.
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