Abstract
Free fatty acids from adipose tissue of rams and wethers slaughtered at 12 and 30 weeks of age were isolated by a modified Bligh and Dyer method and analyzed by gas chromatography−mass spectrometry. A total of 15 free fatty acids were positively identified. At 12 weeks of age, rams had nonsignificantly higher levels of the flavor-significant compounds, 4-methyloctanoic and 4-methylnonanoic acids, than wethers. At 30 weeks of age, differences in the levels of these acids in rams and wethers became significant and 4-methyloctanoic and 4-methylnonanoic acids increased by 13- and 4-fold, respectively, in the noncastrated animals. Log odor unit values indicated that 4-methyloctanoic acid was present at or above its odor threshold value in rams and wethers of both slaughter ages, but that the value was much higher for 30-week-old rams. 4-Methylnonanoic acid was present below its odor threshold value in all samples. Keywords: Branched-chain fatty acids; fatty acids; adipose tissue; lamb; sheepmeat
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