Abstract
The intensity of light emission was used to determine the ground-to-excited state free-energy difference of Photosystem II in the alga Scenedesmus quadricauda. Prompt fluorescence measurements give the driving force of that photosystem under illumination while delayed fluorescence indicates the free energy stored in reaction products. Comparison of the light-on and light-off cases suggested that the immediate products were 0.13 eV lower in free energy than the excited state. In the physiological light range, Photosystem II can store 1.0 eV, or about 60% of that needed for photosynthesis, and still shows a ΔG of 0.7 eV after 3 h.
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