Abstract
Phenolic compounds exist in free and bound forms in cereals. The efficiency, reliability and suitability of recovering free phenolic compounds from barley by conventional, solid–liquid and pressurized solvent extractions, using different mixtures and methods, were tested. The extraction recovery of bound phenolics was evaluated using alkaline and acid hydrolyses. This study illustrates a rapid application of micellar electrokinetic chromatography for the analysis of free and bound phenolic compounds in barley samples. After developing a capillary electrophoresis optimization plan, barley phenols were analyzed within 5.5 min, using a buffer containing 20 mM sodium tetraborate, 10 mM sodium dodecyl sulfate and 5 mM KH 2PO 4 (pH 9), a 40 cm × 50 μm capillary, 30 kV and 30 °C. The selectivity of the extraction methods in recovering phenolic classes was evaluated by capillary electrophoresis and compared with spectrophotometric measurements. Electropherograms of free phenolic extracts showed flavan-3-ol compounds, proanthocyanidins and hydrolysable tannins. Aqueous acetone and aqueous ethanol solvents extracted the highest amount of catechins and hydrolysable tannins, respectively. The extraction yield of bound phenolic compounds (especially hydroxycinnamic acids) increased when the digestion time for alkaline hydrolysis was prolonged.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
