Abstract
CONSIDERABLE research has been conducted during the past eight years attempting to cultivate the chick embryo on a chemically defined medium. Two techniques have been used to expose the early chick embryo in vitro to variations in nutrients. These include Waddington's (1932) classical watch-glass method as modified by Spratt (1947) and New's (1955) method which employs the vitelline membrane to support the embryo. However, attempts to replace the yolk and albumen with a chemically defined medium have not been successful with respect to modifications of the nitrogenous precursors (Klein et al., 1958, 1962); Grau et al., 1959; Hayashi and Herrmann, 1959).It would seem important to know the pattern of the free amino acids in the egg in studies where attempts are made to cultivate chick embryos on a chemically defined medium. One could then pattern the nitrogenous precursors in the chemically defined medium after the free amino acid content .
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