Framework for qPCR modeling and analysis of low copy number sample

Abstract

For the diagnosis of infectious diseases and cancer risk estimation, the importance of accurate quantification of DNA or RNA templates in a sample has been increased. The author utilized the Cq distribution calculation method based on binomial distribution and observed that the distribution is multi-modal, indicating that the distribution cannot be represented by unimodal, such as Log-Normal or Gamma distributions. By combining the result of this model with the Poisson distribution, the Cq values probability distribution for a dilution experiment can be calculate as multi-modal distribution.