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Abstract
Faloak (Sterculia quadrifida R.Br) is one source of bioactive compounds that could be developed as chemotherapeutic agent. Empirically East Nusa Tenggara people use boiled water of faloak bark as a cure for hepatitis (types A, B, and C), and gastroenteritis. This study was performed to test anticancer activity fraction of n-hexane, ethyl acetate, ethanol, and methanol of ethanolic extract from faloak stem bark for the type of breast cancer cell line T47D, and normal cell types Vero using cytotoxic 3- (4,5-dimetilazol- 2-yl) -2,5-difeniltetrazolium bromide (MTT) test method. Ethanolic extract was subjected to column chromatography using different solvents polarity level as n-hexane, ethyl acetate, ethanol, and methanol. Testing the cytotoxic effects using the MTT assay in T47D breast cancer cells and normal Vero cells with EC50 parameter. Ethyl acetate fraction in inducing apoptosis and cell cycle modulation was observed with flowcytometry method. The test results cytotoxic fraction indicating the fraction of ethyl acetate has the lowest activity with EC50 of 24.88 μg/mL and selectivity index of 15.58. Ethyl acetate fraction effects an accumulation of cells in S phase (27.43%) in breast cancer cells T47D which is able to induce apoptosis. These results demonstrate that the ethyl acetate fraction can be developed as a chemotherapeutic agent in improving the effectiveness of breast cancer treatment.
Highlights
Faloak (Sterculia quadrifida R.Br) merupakan salah satu sumber senyawa bioaktif yang dapat dikembangkan sebagai agen obat
These results demonstrate that the ethyl acetate fraction can be developed as a chemotherapeutic agent in improving the effectiveness of breast cancer treatment
G., Gurtu, V., Kain, S.R., dan Yan, G., 1997
Summary
Lima mg fraksi aktif hasil uji sitotoksik dilarutkan dalam kloroform: metanol (1:1), ditotolkan pada plat alumunium silika gel GF254 dan dielusi dengan fase n-propanol:air:asam asetat glasial (5:1:1). Pereaksi semprot yang digunakan antara lain: dragendorff, FeCl3, 2,4-DNPH, vanilin-asam sulfat, anisaldehid-asam sulfat, dan serium sulfat. Setelah perlakuan dengan pereaksi semprot, dilakukan pengamatan sebagai berikut: 1). Induksi apoptosis diketahui dengan membandingkan efek perlakuan senyawa tunggal dan kombinasi dengan kontrol sel. Gambar 2.Efek perlakuan fraksi etil asetat terhadap sel T47D. (a) sel tanpa pelakuan; (b) Fraksi etil asetat dengan konsentrasi 25 μg/mL; Morfologi sel T47D yang hidup ditunjukkan dengan gambar panah Efek perlakuan fraksi etil asetat terhadap sel Vero. (a) sel tanpa pelakuan; (b) Fraksi etil asetat dengan konsentrasi 250 μg/ml Pengamatan dilakukan di bawah mikroskop inverted dengan pembesaran 100x. (a) sel tanpa pelakuan; (b) Fraksi etil asetat dengan konsentrasi 250 μg/ml
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