Abstract
Yellow kepok banana peel (Musa balbisiana) contains antibacterial compounds against S. epidermidis, S. aureus and P.acne. This study aims to proved the antibacterial activity of the yellow kepok banana peel extract fractionated using ethanol as a solvent against S.epidermidis, S.aureus and P.acne. The yellow kepok banana peel extraction process was carried out by maceration method with 70% ethanol followed by fractionation to obtain ethanol fraction. Antibacterial activity testing using nutrient agar (NA) media with wells method with a concentration of 25,000 ppm, 50,000 ppm, 100,000 ppm, aquadest as a negative control and clindamycin as a positive control. The phytochemical test of the ethanol fraction showed the content of alkaloids, flavonoids, saponins, tannins and quinones. The results of the inhibition zone diameter of the ethanol fraction at a concentration of 100,000 ppm against S. epidermidis (11.87 mm), S. aureus (12.04 mm) and P. acne (11.35 mm). The test is to determined the minimum inhibitory concentration (MIC) the minimum inhibitory concentration value of the ethanol fraction of S. epidermidis, S. aureus is 20,000 ppm and for P. acne is 17,500 ppm. The conclusion of this studied was that the ethanol fraction of kepok yellow banana peel extract has inhibitory and bacteriostatic properties against S. epidermidis, S. aureus and P.acne bacteria.Keywords: alkaloids,Ant ibacter ial , flavonoids , Fractionated, Musa balbisiana
Highlights
Yellow kepok banana peel (Musa balbisiana) contains antibacterial compounds against S. epidermidis, S. aureus and P.acne
This study aims to proved the antibacterial activity of the yellow kepok banana peel extract fractionated using ethanol as a solvent against S.epidermidis, S.aureus and P.acne
Endophytic Bacteria Isolates Jurnal Sains dan Matematika,” JSM, vol 22, no
Summary
Efek samping penggunaan antibiotik dapat dikurangi dengan mengganti bahan aktif obat yang diperoleh dari alam seperti kulit buah pisang kepok kuning yang mampu menghambat pertumbuhan bakteri penyebab jerawat. Hasil dari penelitian sebelumnya menunjukan bahwa ekstrak etanol 96% limbah kulit pisang kepok kuning positif memiliki aktifitas sebagai agen antibakteri terhadap bakteri penyebab jerawat (S. epidermidis, S. aureus dan P.acne). Kulit pisang kepok kuning dapat menghambat pertumbuhan bakteri S. aureus [8,2] mm pada konsentrasi 50.000 ppm, S. epidermidis [6,7] mm pada konsentrasi 25.000 ppm dan P.acne [8,4] mm pada konsentrasi 25.000 ppm (1). Berdasarkan dari beberapa hasil uraian tersebut, maka peneliti tertarik melakukan penelitian tentang “Fraksi etanol kulit pisang kepok kuning terhadap bakteri S. epidermidis, S. aureus dan P.acne”
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