Abstract
The segment-specific regulatory domains of the Bithorax complex (BX-C), which consists of three homeotic genes Ubx, abd-A and Abd-B, are separated by boundaries that function as insulators. Most of the boundaries contain binding sites for the architectural protein CTCF, which is conserved for higher eukaryotes. As was shown previously, the CTCF sites determine the insulator activity of the boundaries of the Abd-B regulatory region. In this study, it was shown that fragments of the Fab-3 and Fab-4 boundaries of the abd-A regulatory region, containing CTCF binding sites, are not effective insulators.
Highlights
Chromosome architecture and distant interactions between regulatory elements are one of the key fields of research in modern biology
It was shown previously that DNA fragments Fab-3 (626 bp, F3, (3R:16835097..16834472)) and Fab-4 (784 bp, F4 (3R:16857582..16856799)), despite the presence of dCTCF sites, did not exhibit the properties of insulators at the imago stage in the studies of transgenic Drosophila lines using model regulatory systems of the yellow and white genes [14]
All four sites in the Fab-7attP50 line were removed, which led to premature ectopic activation of the iab-7 domain by the iab-6 initiator in all PS11 cells, as a result of which the A6 segment in males was lysed (Fig. 1b), and in females it was transformed into A7
Summary
Chromosome architecture and distant interactions between regulatory elements are one of the key fields of research in modern biology. These genes are regulated by tissue-specific regulatory domains (abx/bx, bxd/pbx, and iab-2–iab-8), alternating in the order of the location of the segments that they control (Fig. 1a).
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