Fragaria vesca Chlorophyll Mutants

Abstract

an estimated y-irradiation dose of 7.5 krad. M2 and M3 generation seed production resulted from natural self-pollination of M l and M2 plants, respectively, under greenhouse conditions. Among 90 M 2 families grown for evaluation, four contained stable chlorophyll mutants. In families 456, 502, and 648, mutant seedlings (mutants M456, M502, and M648, respectively) had light green, yellow, and white shoots, respectively, and died at the cotyledon or first true leaf stage. Light green seedlings of mutant M538 were viable, grew to sexual maturity, and produced only light green progeny when allowed to self-pollinate in the greenhouse. Normally pigmented siblings in M 2 families 456, 502, 538, and 648 were all fully viable; a proportion (8/13, 6/15, 4/15, and 7/15, respectively) of these normal M 2 plants produced M3 families that segregated for the respective chlorophyll abnormalities, showing that each mutant phenotype was inherited in a recessive manner. When M3 phenotypic segregation patterns