Abstract
DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ϱ = 0.98 ( G + C) + 1.659, where ϱ refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite ( ϱ = 1.686 g/ ml.) and a heavy satellite ( ϱ = 1.712 g/ ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.
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