Abstract
1. 1.|The precipitation of proteins with polyethylene glycol has been studied with a view to determining the effect of protein concentration and other conditions on the separation. Assuming the process to be one of colloid phase separation, the expression log S=K−βω where S is the solubility of the protein, ω the concentration of the polymer and β and K constants was derived to describe the process. 2. 2.|The derivation showed that the slope, β, should increase with the size of the protein molecule and be otherwise unaffected by conditions. 3. 3.|This indicated that the technique can be treated quantitatively the same as the wellknown salting-out technique, and that its selectivity should be greater for larger particles such as viruses. 4. 4.|The precipitation of the proteins carboxyhaemoglobin, ovalbumin and bovine serum albumin, as well as brome grass mosaic virus were found to conform with the above equation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Protein Structure
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
