Fractionation of Labisia Pumila using Solid-Phase Extraction for Extraction of Gallic Acid

Abstract

Phenolics are the most ample secondary metabolites of plants and have drawn increasing attention in their applications in health supplement and functional cosmetic. This is due to their strong antioxidant properties and their marked effects in the inhibition of several oxidative stress associated metabolic disorder such as cancer and aging. In Labisia pumila, gallic acid (3,4,5-Trihydroxybenzoic acid) was found to be one of the most abundant secondary metabolite. It has been proposed as a biomarker in cosmetic and food supplement applications. In this research the identification and isolation of gallic acid were carried out. Different sorbent of solid-phase extraction (SPE) column were used for the fractionation of Labisia pumila var Alata water extract and the amount of gallic acid present in the fractions were quantified. The aim is to identify the better sorbent-solvent combination in SPE that result in higher recovery of gallic acid from Labisia pumila var Alata water extract. A range of solvent mixtures of 20%, 40%, 60% and 80% methanol (Me Oh)-water was used. Gallic acid was successfully extracted and identified from Labisia pumila. From this discovery, C18 silica reversed phase solid phase extraction column could extracts highest gallic acid from Labisia pumila var Alata water extract. Retention of analyte from the sample from second elution using 80% methanol-water Solvent may be used as the best method to extract the compound from Labisia Pumila var Alata since it has been reported to have various bioactivities such as high antioxidant activity and cytotoxic against many diseases. Using C18 silica reversed phase in SPE as a fractionation and extraction of gallic acid from Labisia pumila var Alata can be achieved for application purpose or future studies.