Abstract
Three day plaque from fasting subjects was fractionated into cellular and acellular components by a number of preparative procedures. The cellular fraction was separated into two sub-fractions, one containing most of the plaque microorganisms, the other containing the mammalian cells and clumps of microorganisms which could not be separated. Fifty per cent of the acellular fraction consisted of material of molecular weight greater than 100,000. The low molecular weight material in the remainder probably originated from the cytoplasm of the plaque cells. The high molecular weight portion of the acellular fraction, comprising 36 per cent of the total plaque, was separated during fractionation into two components, a precipitate and supernatant. These contained six and five sub-fractions respectively. Carbohydrate was present in varying amounts in each of the various fractions and sub-fractions and calcium and phosphate were present in most. Amino acid analysis of the high molecular weight portion of the acellular fraction suggested that a large part of this fraction was from salivary mucin although other data indicated the presence also of nucleic acid. The isoelectric point of the supernatant portion of this fraction was lower than that of the precipitate portion (2.7 and 3.2 respectively).
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