Abstract
This paper reports the first application of a combined approach utilizing split-flow thin-cell (SPLITT) separation to size fractionate natural aquatic colloids and particles collected from freshwater samples. No sample preconcentration was performed although some samples were investigated after alteration of the ambient pH. The unfractionated and fractionated samples were analyzed by scanning electron microscopy (SEM), environmental SEM, and 3D excitation emission matrix fluorescence. Qualitative and quantitative results by microscopy indicated that SPLITT produces well-resolved fractionations at appropriate sizes but with some perturbation of the sample. In addition, tryptophan-like fluorescence was shown to be caused by different organic moieties compared with humic-like and fulvic-like fluorescence. Tryptophan-like fluorescence intensity is found mainly in the particulate material but is not pH dependent, while humic- and fulvic-like fluorescence intensities are dependent on pH but not on size. Fulvic-like fluorescence intensity normalized to absorbance, related to fluorescence efficiency and molar mass, varies with size.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
