Abstract
Complementary RNA molecules or fragments of denatured DNA can be fractionated by means of temperature or salt concentration gradients applied to columns of DNA-agar which contain RNA-DNA or DNA-DNA duplexes. The guanine + cytosine content of eluted RNA molecules is proportional to the elution temperature. The stability of RNA-DNA duplexes is lower than that of DNA-DNA duplexes when subjected to increasing temperatures or decreasing ionic strength. Some physical-chemical and biological implications of the results are discussed.
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