Abstract
SUMMARYA Tris buffer system (starting buffer, 0.04M Tris phosphate, pH 9.0; limiting buffer, 0.5M Tris H2PO4, pH 3.6) and a concave gradient elution procedure were developed for the fractionation of beef sarcoplasmic proteins by diethylaminoethyl cellulose ion‐exchange chromatography. Sarcoplasmic proteins extracted 2 hr post‐mortem were separated chromatographically into 16–18 distinct fractions. Duplication of the results was excellent. Some alterations occurring in these proteins during 10 days' post‐mortem aging were detected by this technique. Such changes observed were the disappearance of one fraction and appearance of new components, while other fractions diminished.
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