Abstract
The 34 S RNA subunits of three RNA tumor viruses (virus from spontaneously activated AKR cells, Moloney leukemia virus, and RD114 virus) were examinated for differences in poly (A) content. Poly(U)-Sepharose columns and a temperature elution regime were used to fractionate the sub-units. The 34 S RNA of the two murine viruses characteristically chromatographs into two fractions. One fraction, which constitutes 30 to 40% of the RNA, contains no poly(A) regions longer than 20 nucleotides. A second fraction contains poly(A) stretches of 150 to 200 nucleotides and constitutes 60 to 70% of the viral 34 S RNA. In contrast, the RD114 viral RNA can be resolved into three fractions. Approximately 60% is not retained by the column and contains no poly(A) regions larger than 20 nucleotides. Of the viral RNA that is bound to the column, 28% elutes with characteristics of RNA having poly(A) stretches of 50 to 75 nucleotides and 12% elutes with characteristics of RNA having poly(A) stretches of 100 to 150 nucleotides. These results indicate that the viral 34 S subunits are heterogeneous with respect to their poly(A) contents and that there may be significant differences among RNA viruses with respect to the size and distribution of their poly(A) regions.
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