Abstract

The control variables involved in the extraction of seed protein from Terminalia catapa seed meal have being investigated. These include extraction time, pH, solid-solvent ratio. Protein and fractionated into albumin (4.25%), glutelin (3.57%), prolamin (8.96%) and globulin (6.35%). The extraction time (min) of 15, 30, 45 and 60 were used for fractionation of the seed protein. The results showed an increase in protein yield across the fractions with increasing extraction time; highest protein yield of 10.2% was recorded in prolamin at 60 mins. The pH dependent solubility profile revealed that the region of iso-electric point (minimum solubility) was at pH 8.0, the solubility reduced as the pH increased until it reached the iso-electric point which was followed by progressive increase in solubility with further increase in pH. The solid-solvent ratio of 1:5, 1:10, 1:20, 1:30 and 1:40 were used and the result shows that there was increase across the fraction with increasing solid-solvent ratio. Highest protein yield was recorded at ratio 1:40

Highlights

  • Due to the exorbitant cost of protein from animal sources has led to a growing interest in industrial application of protein from plant origin to challenge the increasing demand for protein in food and non-food market

  • The present study aims at reporting the protein content and optimum extraction conditions of Nigerian Tropical almond (Terminalia catapa) seed

  • Statistical analysis Data generated from this study were subjected to Analysis of variance (ANOVA) and means were separated by the Duncan multiple range test (DMRT) using the Statistical package for Social Sciences version16.0 for windows (SPSS Inc., Illinois, USA)

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Summary

Current Research in Nutrition and Food Science

Fractionation and Some Conditions on the Extract Ability of Tropical Almond (Terminalia catapa) Seed Protein. The control variables involved in the extraction of seed protein from Terminalia catapa seed meal have being investigated. These include extraction time, pH, solid-solvent ratio. The extraction time (min) of 15, 30, 45 and 60 were used for fractionation of the seed protein. The results showed an increase in protein yield across the fractions with increasing extraction time; highest protein yield of 10.2% was recorded in prolamin at 60 mins. The solid-solvent ratio of 1:5, 1:10, 1:20, 1:30 and 1:40 were used and the result shows that there was increase across the fraction with increasing solid-solvent ratio.

INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
Defatted meal
Albumin Glutelin Prolamin Globulin
Full Text
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