Abstract
Qualitative analysis of three groups of antibiotics, tetracyclines, macrolides and aminoglycosides, was carried out in acid buffer solution at the 0.5ppm level, except for hygromycin B (10ppm) and destomycin A (2ppm), as a preliminary to residue analysis studies on animal tissues.Macrolides and tetracyclines were separated from aminoglycosides by extraction with chloroform at pH 8.50 and then with ethyl acetate at pH 5.50.After extraction, aminoglycosides in buffer solution were freed from residual tetracyclines by treatment with Amberlite XAD-2 at pH 4.00.Each fraction was subjected to microbioautography for the identification of antibiotics.
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