Abstract
Data on fractional composition of proteins and peptides in the dry fermentolizate and starter feed developed in TINRO are presented and compared with that composition in natural prey consumed by salmon juveniles and in compound feed for them used in fish farms. The dry fermentolizate is produced on base of raw pollock with successive processes of grinding, fermentation (45 min. under temperature 45–50 о C with complex enzyme preparation Protosubtilin G3x), bone separation, and pasteurization (15–20 min. under temperature 85–90 о C). The starter feed includes fish flour (pollock), wheat flour, dry fermentolizate, bran, hydrolysis yeast, milk powder, premix, and vegetable oil in a certain proportion. Methods of fractional composition investigation for the water-soluble part of protein component in feeding products are described in detail. The fractional composition is determined using the medium pressure gel permeation chromatography. General chemical composition of the high-protein dry fermentolizate and starter feed is presented. Contents of polypeptides and low molecular weight proteins with MM of 1.0–10.0 kDa, proteins with MM of 0.1–10.0 kDa and > 10.0 kDa for the new-developed starter feed with addition of dry fermentolizate coincide with those for natural prey of salmon larvae and juveniles (chironomids, oligochaetes, gammarus), so it corresponds to real nutritional needs of juvenile salmon.
Highlights
Проведен анализ фракционного состава белков и пептидов сухого ферментолизата и стартового комбикорма разработки Тихоокеанский филиал ВНИРО (ТИНРО) в сравнении с составом кормовых организмов молоди лососевых и комбикормом для них
The dry fermentolizate is produced on base of raw pollock with successive processes of grinding, fermentation (45 min. under temperature 45–50 оC with complex enzyme preparation Protosubtilin G3x), bone separation, and pasteurization (15–20 min. under temperature 85–90 оC)
Methods of fractional composition investigation for the water-soluble part of protein component in feeding products are described in detail
Summary
Проведен анализ фракционного состава белков и пептидов сухого ферментолизата и стартового комбикорма разработки ТИНРО в сравнении с составом кормовых организмов молоди лососевых и комбикормом для них. Установлено, что фракционный состав стартового комбикорма ЛСоТ разработки ТИНРО с добавлением сухого ферментолизата по фракционному составу белка и пептидов сопоставим с реальными потребностями молоди кеты. Bashtovoy A.N., Karaulova E.P., Timchishina G.N., Pavel K.G., Pasechnik P.L. Fractional composition of water-soluble protein components of dry fermentolizate and starter feed for salmon fish // Izv. TINRO. Цель статьи — описать фракционный состав водорастворимых белков и пептидов экспериментального стартового комбикорма ЛСоТ, разработанного в ТИНРО, и его составной части — сухого ферментолизата, а также сопоставить полученные данные с имеющимися в литературе данными [Пономарев, Пономарева, 2003] по фракционному составу кормовых организмов для молоди тихоокеанских лососевых
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