Fractional changes of pectic polysaccharides in different tissue zones of developing guava ( Psidium guajava L.) fruits

Abstract

Cell-wall pectic polysaccharides were fractionated by sequential extraction with water, 1% potassium oxalate at room temperature, 0.05 M HCl at 100 °C and 0.05 M NaOH at 4 °C from the three specific tissue zones, i.e., outer pericarp (OP), middle pericarp (MP) and inner pericarp (IP) of the guava fruits during their development and ripening. The water and oxalate-soluble pectic fractions were found to increase, while acid and alkali-soluble pectic fractions had a decreasing trend at ripening phase of fruit, irrespective of the different tissue zones. However, the extent of pectic solubilization was more pronounced in IP region as compared to OP and MP zone of the fruit tissue. The differential degradation of pectic polymers thus points out that ripening as well as tissue softening of guava fruits are centrifugally expressed as evidenced by higher accumulation of sugar and soluble pectic polysaccharides in IP zone of the fruits.