Fraction Activity Test of Suon Stem Bark (Datura Sp.) against Staphylococcus Aureus, Staphylococcus Epidermidis in Vitro

Abstract

The stem bark of Suon (Datura sp.) is one of the Papuan endemic plants. It is used as a traditional medicinal plant as an antimalarial and new or old wound medicine by the Yali Tribe in Sabelebi Village, Heriapini District, Yahukimo Regency by wrapping the young and flexible stem bark of Suon in the wound area. This plant has secondary metabolite compounds potential as an antibacterial, such as flavonoids, tannins, alkaloids, and saponins. This research aimed to determine the inhibition of antibacterial activity and the most effective concentration in inhibiting Staphylococcus aureus and Staphylococcus epidermidis bacteria. The research sample used the stem bark of Suon obtained from Sabelebi village, Yahukimo regency. Samples were extracted by maceration method using 96% ethanol solvent, then fractionated using n-Hexane, Ethyl-acetate, and Ethanol as solvents. Antibacterial activity test using disc diffusion method. The results of the Suon stem bark fraction (Datura sp.) against Staphylococcus aureus bacteria have the most effective inhibitory power and concentration, the ethanol-water fraction 50 ppm, the ethyl acetate fraction 50 ppm, and the n-Hexane fraction 50 ppm. Furthermore, Staphylococcus epidermidis have the most effective inhibitory power and concentration, the ethanol-water fraction 50 ppm, the ethyl acetate fraction 50 ppm, and the n-Hexane fraction 50 ppm. From the test results, it can conclude that the stem bark of Suon (Datura sp.) potentially inhibits Staphylococcus aureus and Staphylococcus epidermidis bacteria.