Fractalkine对类风湿关节炎患者成纤维样滑膜细胞中 NF-κB活化及内源性fractalkine mRNA表达的影响

Abstract

AIM: To investigate the effects of fractalkine (FKN) on nuclear factor kappa B (NF-κB) activation and endogenous FKN mRNA expression in fibroblast-like synoviocytes (FLS) from the patients with rheumatoid arthritis (RA). METHODS: RA-FLS were gained through tissue culture. Fractalkine at 100 μg/L was used to stimulate RA-FLS for 0 h, 1 h and 2 h. The expression of NF-κB p65 protein in cytoplasm and nucleus was detected by Western blotting, representing the activation of NF-κB in RA-FLS. RA-FLS was stimulated with fractalkine at concentration of 100 μg/L for 0 h, 12 h or 18 h, and the mRNA expression of FKN in RA-FLS was detected by RT-PCR. RESULTS: After stimulated with recombinant human FKN for 1 h, the expression of NF-κB p65 protein in the cytoplasm of RA-FLS was obviously lower than that in RA-FLS without FKN treatment in control group (P＜0.05). After stimulated with FKN for 2 h, the expression of NF-κBp65 protein in nucleus was obviously higher than that in RA-FLS of control group (P＜0.05). Recombinant human FKN at concentration of 100 μg/L induced endogenous FKN mRNA expression in RA-FLS in a time-dependent manner. The mRNA expression of FKN in RA-FLS obviously increased after stimulated with FKN for 18 h (P＜0.05). CONCLUSION: FKN up-regulates the expression of endogenous FKN mRNA, suggesting a positive feedback. FKN can activate the NF-κB and may play an important role in the beginning of joint inflammation, angiogenesis and bone destruction.