Fractal-based aberration-corrected full-field OCT.

Abstract

The Kolmogorov turbulence model has been validated as a quantitative 3D light scattering model of the inhomogeneous refraction index of biological tissue using full-field OCT (FF-OCT). A fractal-based computational compensation approach was proposed for correcting of depth-resolved aberrations with volumetric FF-OCT. First, the power-spectral density spectrum of the index inhomogeneities was measured by radial Fourier transformation of volumetric data. The spectrum's shape indicates the spatial correlation function and can be quantified as the fractal dimension of tissue. The defocusing correction matrix was built by applying fractal-based analysis as an image quality metric. For comparison, tissue-induced in-depth aberration models were built by phase compensation. After digital aberration correction of FF-OCT images, it enables extracting the temporal contrast indicating the sample dynamics in onion in mitosis and ex vivo mouse heart during delayed neuronal death. The proposed fractal-based contrast augmented images show subcellular resolution recording of dynamic scatters of the growing-up onion cell wall and some micro activities. In addition, low-frequency chamber and high-frequency cardiac muscle fibers from ex vivo mouse heart tissue. Therefore, the depth-resolved changes in fractal parameters may be regarded as a quantitative indicator of defocus aberration compensation. Also the enhanced temporal contrast in FF-OCT has the potential to be a label-free, non-invasive, and three-dimensional imaging tool to investigate sub-cellular activities in metabolism studies.