Abstract
BackgroundForkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family, and it has recently been found to participate in cancer development. However, whether FoxQ1 expression contributes to glioma development and progression is not known. We investigate FoxQ1 expression in gliomas and the role of FoxQ1 during tumorgenesis.MethodsReverse transcription quantitative real-time PCR (RT-qPCR) and Western blot were used to determine the FoxQ1 and Neurexins 3 (NRXN3) expression in gliomas. Chromatin immunoprecipitation (ChIP) and luciferase assays were used to determine the regulation between FoxQ1 and NRXN3. We established depleted FoxQ1 stable clones in U-87MG cells and overexpressed FoxQ1 stable clones in SW1088 cells. MTT and transwell were used to evaluate the ability of proliferation and migration, respectively.ResultsFoxQ1 mRNA and protein were up-regulated in gliomas and negatively related to the NRXN3 expression (r = −0.373, P = 0.042). FoxQ1 directly binds to NRXN3 promoter region and suppresses the promoter activity. Furthermore, the ability of proliferation and migration is reduced in depleted FoxQ1 cells.ConclusionFoxQ1 promotes glioma cell proliferation and migration by down-regulation of NRXN3 expression.
Highlights
Gliomas are the most common tumors of the central nervous system in adults
We showed that Forkhead box Q1 (FoxQ1) expression was higher in glioma specimens than the normal tissues, whereas NRXN3 expression was lower in glioma specimens
FoxQ1 is overexpressed in human glioblastomas and negatively correlates with NRXN3 expression
Summary
Gliomas are the most common tumors of the central nervous system in adults. Patients with glioblastoma routinely undergo surgery followed by adjuvant radiation therapy and chemotherapy. Diagnosis and therapeutic strategies have been progressed, the median survival times still less than 1 year in most cases. The 5-year survival rate in patients with glioma is among the lowest for all cancers [1,2]. The understandings of the biology and molecular mechanisms of glioma development and progression are not well known. The forkhead box (Fox) gene family is a large and diverse group of transcription factor and plays important roles in biological processes, including development, metabolism, immunology, and senescence [3,4]. Forkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family, and it has recently been found to participate in cancer development. Whether FoxQ1 expression contributes to glioma development and progression is not known. We investigate FoxQ1 expression in gliomas and the role of FoxQ1 during tumorgenesis
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