FOXM1/LINC00152 feedback loop regulates proliferation and apoptosis in rheumatoid arthritis fibroblast-like synoviocytes via Wnt/β-catenin signaling pathway.

Wenlong Wang,Long He,Mengjie Chen,Piaopiao Guo,Yongjun Cheng,Die Chen

doi:10.1042/bsr20191900

Wenlong Wang, Long He + Show 4 more

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https://doi.org/10.1042/bsr20191900

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Journal: Bioscience reports	Publication Date: Jan 21, 2020
Citations: 26	License type: CC BY 4.0

Affiliation: The First People's Hospital of Wenling

Abstract

Rheumatoid arthritis (RA), a chronic systemic disease, is featured with inflammatory synovitis, which can lead to destruction on bone and cartilage and even cause disability. Emerging studies demonstrated that Fibroblast-like synoviocytes (FLS) is a vital cellular participant in RA progression. Long non-coding RNAs (lncRNAs) are also reported to participate in the pathogenesis of RA. In our present study, lncRNA microarray analysis was applied to screen out lncRNAs differentially expressed in RA FLS. Among which, cytoskeleton regulator RNA (LINC00152) presented biggest fold change. Gain- or loss-of function assays were further carried out in RA FLS, and the results revealed that LINC00152 promoted proliferation but induced apoptosis in RA FLS. Furthermore, up-regulation of LINC00152 may induce promotion of Wnt/β-catenin signaling pathway in RA FLS. Mechanistically, we found that forkhead box M1 (FOXM1) transcriptionally activated LINC00152 in RA FLS. Additionally, LINC00152 positively regulated FOXM1 via sponging miR-1270. In conclusion, the present study focused on elucidating the function of FOXM1/LINC00152 positive feedback loop in RA FLS and its association with Wnt/β-catenin signaling.

Highlights

Rheumatoid arthritis (RA), a chronic systemic disease, is featured with inflammatory synovitis, which can lead to destruction on bone and cartilage and even cause disability [1,2]
To screen out RA-associated Long non-coding RNAs (lncRNAs), we applied a microarray analysis to find lncRNAs that were differentially expressed in RA Fibroblast-like synoviocytes (FLS)
Up-regulation of LINC00152 in RA FLS was proved by qRT-PCR (Figure 1B)

Summary

Introduction

Rheumatoid arthritis (RA), a chronic systemic disease, is featured with inflammatory synovitis, which can lead to destruction on bone and cartilage and even cause disability [1,2]. Deep understanding of the molecular mechanism involved in RA development is necessary to find novel efficient diagnostic method

Methods

Results

Conclusion