Abstract

The Chinese alligator (Alligator sinensis) is one of the most endangered crocodilians in the world. In this study, 14 novel microsatellite loci were developed for this rare species via 454 high-throughput sequencing. These polymorphic microsatellites yielded an average of 2.93 alleles per locus among 40 individuals scored. The observed and expected heterozygosities (H O and H E ) ranged from 0.025 to 0.975 and from 0.025 to 0.651, with averages of 0.439 and 0.426, respectively. These microsatellite markers will be useful for individual identification, mate choice for captive breeding and other relevant genetic investigations of Chinese alligator.

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