Abstract
Background: Dental calculus harbours microbes responsible for periodontal disease. Prevention of it formation prevents tooth loss. The composition of dental calculus may serve as vital clue to its preventive strategy. Aims: To assess the compositional differences in supragingival and subgingival human dental calculus by using infrared spectroscopy. Materials and method: Supra gingival and subgingival calculus samples at specified intraoral locations were obtained from human subjects. Samples from twenty patients were analysed using Fourier Transform Infrared Spectroscopy (FTIR). Obtained results were analyzed and compared. Results and conclusion: Characteristic spectrum and wave-peaks of supragingival and subgingival calculus from various sites were similar with no significant differences among them. There was a statistically significant difference (p=0.027) among the wave peaks of only in the region which is characteristic of functional groups with double bonds
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