Abstract
A phytochemical analysis has been carried out on the glycoside-enriched fraction of a MeOH extract of Helleborus orientalis rhizomes, resulting in the isolation of two new bisdesmosidic furostanol saponins (1 and 2) and two new furospirostanol saponins (3 and 4). The structures of 1-4 were determined on the basis of extensive spectroscopic analysis and acid hydrolysis followed by chromatographic and spectroscopic analysis. Compound (3) showed moderate cytotoxic activity against HSC-2 human squamous cell carcinoma cells with an IC 5 0 value of 16 μg/mL.
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